Composite

Part:BBa_K3002202:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


L2 spectinomycin resistance + cCA_Mut-PETase + cCA_MHETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6639
    Illegal PstI site found at 6963
    Illegal PstI site found at 7306
    Illegal PstI site found at 8116
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal NheI site found at 2665
    Illegal NheI site found at 5561
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6639
    Illegal PstI site found at 6963
    Illegal PstI site found at 7306
    Illegal PstI site found at 8116
    Illegal NotI site found at 6974
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal BglII site found at 7884
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6639
    Illegal PstI site found at 6963
    Illegal PstI site found at 7306
    Illegal PstI site found at 8116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6639
    Illegal PstI site found at 6963
    Illegal PstI site found at 7306
    Illegal PstI site found at 8116
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3220
    Illegal NgoMIV site found at 3247
    Illegal NgoMIV site found at 4898
    Illegal NgoMIV site found at 6572
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.


Source

This part is a combination of the parts BBa_K3002102 (spectinomycin resistance), BBa_K3002106 (cCA_Mutant PETase) and BBa_K3002113 (cCA_wildtype MHETase).

References